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Production of Organic Acids

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...

Ion Exchange

Ion Exchange

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...

Ion-Exchange Chromatography

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...

Production of Alcohol

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Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...

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Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System

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Extractive lactic acid fermentation using ion-exchange resin.

A Srivastava¹, P K Roychoudhury, V Sahai

¹Biochemical Engineering Research Centre, Indian Institute of Technology, Hauzkhas, New Delhi 110016, India.

Biotechnology and Bioengineering

|March 15, 1992

Summary

This summary is machine-generated.

Extractive fermentation using ion-exchange resin significantly enhances lactic acid production. This technique overcomes end-product inhibition, boosting yield and productivity compared to conventional methods.

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Area of Science:

Biotechnology
Biochemical Engineering
Industrial Microbiology

Background:

Lactic acid fermentation is crucial for various industries but is limited by end-product inhibition.
Conventional batch fermentation restricts lactic acid yield and productivity due to product accumulation.

Purpose of the Study:

To investigate the efficacy of extractive fermentation using ion-exchange resin to overcome lactic acid inhibition.
To enhance lactic acid yield and productivity through continuous product removal.

Main Methods:

An ion-exchange resin packed column was integrated with a 2-L fermentor for in-situ lactic acid separation.
Comparative analysis of lactic acid fermentation in conventional batch mode versus extractive mode.
Evaluation of the impact of temperature on extractive fermentation performance.

Main Results:

Conventional batch fermentation achieved a lactic acid yield of 0.828 g/g and productivity of 0.313 g/L/h.
Extractive fermentation significantly improved yield to 0.929 g/g and productivity to 1.665 g/L/h.
Temperature demonstrated a remarkable effect on the efficiency of extractive fermentation.

Conclusions:

Extractive fermentation is a highly effective strategy to overcome end-product inhibition in lactic acid production.
The integrated ion-exchange resin system offers a substantial improvement in fermentation efficiency.
Optimizing temperature is critical for maximizing the benefits of extractive fermentation for lactic acid biosynthesis.