Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...

Double Resonance Techniques: Overview

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...

Nuclear Overhauser Enhancement (NOE)

Nuclear Overhauser Enhancement (NOE)

Irradiation of a spin-active nucleus causes an increase or decrease in the signal intensity of neighboring nuclei that are not necessarily chemically bonded or involved in J-coupling. This phenomenon, called the nuclear Overhauser enhancement (NOE), results from through-space interactions between the nuclear spins. The NOE effect decreases with increasing internuclear distance and is generally not observed beyond 4 angstroms. In NOE, dipole-dipole interactions between neighboring spin-active...

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...

¹³C NMR: ¹H–¹³C Decoupling

¹³C NMR: ¹H–¹³C Decoupling

The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...

Atomic Nuclei: Nuclear Relaxation Processes

Atomic Nuclei: Nuclear Relaxation Processes

In the absence of an external magnetic field, nuclear spin states are degenerate and randomly oriented. When a magnetic field is applied, the spins begin to precess and orient themselves along (lower energy) or against (higher energy) the direction of the field. At equilibrium, a slight excess population of spins exists in the lower energy state. Because the direction of the magnetic field is fixed as the z-axis, the precessing magnetic moments are randomly oriented around the z-axis. This...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An experimental study of triplet spin-lattice relaxation in the system naphthalene-pentacene.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2026

Same author

Overhauser effects in non-conducting solids at 1.2 K.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2017

Same author

Cubic three-dimensional hybrid silica solids for nuclear hyperpolarization.

Chemical science·2017

Same author

Investigation of Solid D 2 for UCN Sources.

Journal of research of the National Institute of Standards and Technology·2016

Same author

An apparatus for pulsed ESR and DNP experiments using optically excited triplet states down to liquid helium temperatures.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2013

Same author

Magnetotransport properties depending on the nanostructure of Fe(3)O(4) nanowires.

Journal of physics. Condensed matter : an Institute of Physics journal·2011

Search research articles

Related Experiment Video

Updated: Jul 3, 2026

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

A 140 GHz prepolarizer for dissolution dynamic nuclear polarization.

S Jannin¹, A Comment, F Kurdzesau

¹Institut de Physique des Nanostructures, Ecole Polytechnique Federale de Lausanne, Station 3, CH-1015 Lausanne, Switzerland.

The Journal of Chemical Physics

|July 8, 2008

Summary

Dynamic nuclear polarization (DNP) enhances sensitivity in high-resolution NMR. This study demonstrates that dissolution-DNP with conventional radicals maintains signal enhancement at magnetic fields up to 5 Tesla.

More Related Videos

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Hyperpolarized ¹³C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate

Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-¹³C]pyruvate

Published on: September 13, 2019

Related Experiment Videos

Last Updated: Jul 3, 2026

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Dissolution Dynamic Nuclear Polarization Instrumentation for Real-time Enzymatic Reaction Rate Measurements by NMR

Published on: February 23, 2016

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging

Hyperpolarized ¹³C Metabolic Magnetic Resonance Spectroscopy and Imaging

Published on: December 30, 2016

Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-13C]pyruvate

Measuring the Spin-Lattice Relaxation Magnetic Field Dependence of Hyperpolarized [1-¹³C]pyruvate

Published on: September 13, 2019

Area of Science:

Nuclear Magnetic Resonance (NMR) Spectroscopy
Magnetic Resonance Imaging (MRI)
Physical Chemistry

Background:

Dynamic nuclear polarization (DNP) is traditionally used for polarized targets in particle physics.
DNP has emerged as a crucial technique for enhancing sensitivity in high-resolution NMR spectroscopy.
A common assumption is that DNP's effectiveness diminishes at higher magnetic fields.

Purpose of the Study:

To investigate the impact of high magnetic fields on DNP-enhanced NMR sensitivity.
To evaluate the performance of dissolution-DNP using conventional radicals at elevated field strengths.

Main Methods:

Employed dissolution-dynamic nuclear polarization (DNP).
Utilized conventional 2,2,6,6-tetramethylpiperidine 1-oxyl (nitroxide) radicals as the paramagnetic agent.
Conducted experiments at magnetic fields up to 5 Tesla.

Main Results:

Demonstrated that signal enhancement in dissolution-DNP does not deteriorate at higher magnetic fields.
Confirmed the efficacy of DNP for sensitivity enhancement in high-resolution NMR (solid and liquid states) up to 5 T.
Showcased the utility of conventional nitroxide radicals in maintaining DNP performance.

Conclusions:

Dissolution-DNP with conventional radicals is effective for NMR sensitivity enhancement at fields up to 5 T.
Challenges the notion that DNP performance degrades significantly at higher magnetic fields.
Highlights the potential of DNP for advanced NMR applications in various states of matter.