Comparative evaluation of test parameters in plaque removal. A preliminary report

A comparative study was conducted to determine the effect of several parameters on the removal of simulated plaque. Streptococcus mutans grown in a 2% sucrose medium was allowed to deposit on porous ceramic tiles in the course of the culture growth to form simulated plaque. The tiles with the culture deposit were dried and stained with Safranin O stain, then used in the plaque removal studies. The parameters evaluated in the course of the study were (a) flowing cold water vs flowing hot water and (b) brushing with toothpaste alone vs brushing with hot water as well as brushing alone. It was observed that flowing hot water was more effective than flowing cold water in the removal of the simulated plaque. Brushing with hot water was found to be a more efficient means of removing the plaque than brushing with toothpaste or brushing alone. The study suggests that it is more effective to use hot water for plaque removal in the course of performing daily oral hygiene.