Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Glycocalyx and its Functions01:14

Glycocalyx and its Functions

The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
Components of...
Membrane Carbohydrates01:30

Membrane Carbohydrates

The plasma membrane is a dynamic barrier composed of lipids, proteins, and carbohydrates. It is the epicenter of many cellular processes required for cell growth and survival. Carbohydrates have unique structural and chemical properties that help the plasma membrane to carry out its functions effectively.
Membrane carbohydrates do not have any hydrophobic region and are exclusively located on the cell's outer surface. The addition of sugar molecules or glycosylation of proteins happens in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modular pentameric protein scaffold based on glutaraldehyde-crosslinked Stx1B for superior multivalent therapeutics.

International journal of biological macromolecules·2026
Same author

Sialic Acid-Containing Glycolipids Extend the Receptor Repertoire of Enterovirus-D68.

ACS infectious diseases·2025
Same author

Multivalent Siglec ligands.

Carbohydrate research·2025
Same author

Kinomic profiling to predict sunitinib response of patients with metastasized clear cell Renal Cell Carcinoma.

Neoplasia (New York, N.Y.)·2024
Same author

Silicon-Based Linkers for Tunable Acid-Sensitive Drug Release from Polymeric Nanoparticles.

Chemistry (Weinheim an der Bergstrasse, Germany)·2024
Same author

Targeting <i>N</i>-Acetylglucosaminidase in <i>Staphylococcus aureus</i> with Iminosugar Inhibitors.

Antibiotics (Basel, Switzerland)·2024

Related Experiment Video

Updated: Jul 3, 2026

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles
14:37

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles

Published on: July 6, 2012

Multivalent carbohydrate recognition on a glycodendrimer-functionalized flow-through chip.

Hilbert M Branderhorst1, Rob Ruijtenbeek, Rob M J Liskamp

  • 1Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P.O. Box 80082, 3508 TB Utrecht, The Netherlands.

Chembiochem : a European Journal of Chemical Biology
|July 8, 2008
PubMed
Summary
This summary is machine-generated.

This study demonstrates a novel microarray of glycodendrimers for real-time analysis of lectin binding. The system effectively visualizes multivalency enhancement, offering a valuable tool for studying molecular interactions and kinetics.

More Related Videos

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Engineering Antiviral Agents via Surface Plasmon Resonance
13:00

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

Related Experiment Videos

Last Updated: Jul 3, 2026

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles
14:37

High-throughput Synthesis of Carbohydrates and Functionalization of Polyanhydride Nanoparticles

Published on: July 6, 2012

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Engineering Antiviral Agents via Surface Plasmon Resonance
13:00

Engineering Antiviral Agents via Surface Plasmon Resonance

Published on: June 14, 2022

Area of Science:

  • Carbohydrate chemistry
  • Biophysics
  • Materials science

Background:

  • Dendrimers are branched macromolecules with tunable properties.
  • Glycodendrimers display multiple carbohydrate moieties, mimicking biological structures.
  • Understanding multivalency effects is crucial for molecular recognition studies.

Purpose of the Study:

  • To develop a microarray of glycodendrimers for studying carbohydrate-lectin interactions.
  • To investigate the multivalency enhancement (cluster effect) in real-time.
  • To assess the utility of the platform for kinetic and thermodynamic analysis.

Main Methods:

  • Synthesis of mannose-functionalized dendrimers using click chemistry.
  • Immobilization of glycodendrimers onto aluminum oxide chips via a spacer.
  • Real-time monitoring of fluorescent lectin binding (ConA, GNA) using the microarray.
  • Inhibition studies with alpha-methyl mannose.

Main Results:

  • Successful creation of a glycodendrimer microarray.
  • Real-time observation of lectin binding and multivalency enhancement.
  • Differential cluster effects observed for ConA (small) and GNA (large), correlating with binding site spacing.
  • Demonstration of inhibition experiments for kinetic analysis.

Conclusions:

  • The glycodendrimer microarray is an effective platform for studying multivalency effects in carbohydrate-lectin interactions.
  • The system allows for real-time kinetic and thermodynamic data acquisition.
  • This technology serves as a valuable screening tool for molecular binding events.