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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jul 3, 2026

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Flow Cytometry Protocols for Surface and Intracellular Antigen Analyses of Neural Cell Types

Published on: December 18, 2014

Quantification of surface antigens and quantitative flow cytometry.

Huai En Huang Chan¹, Iman Jilani, Richard Chang

¹Department of Hematopathology, Quest Diagnostics Nichols Institute, San Juan Capistrano, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|July 9, 2008

Summary

Quantifying cell surface antigens like CD20 is key for diagnosing B-cell chronic lymphocytic leukemia and monitoring antibody therapies. This study presents a flow cytometry method to precisely measure these antigen levels for better treatment management.

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Area of Science:

Immunology
Oncology
Biotechnology

Background:

Accurate measurement of cell surface antigens is crucial for diagnosing diseases like B-cell chronic lymphocytic leukemia.
Monitoring antigen levels is vital for assessing the efficacy of antibody-based therapies.

Purpose of the Study:

To develop and describe a quantitative flow cytometry method for measuring cell surface antigen expression.
To enable precise quantitation of molecules like CD20 on cell surfaces for therapy monitoring.

Main Methods:

Utilized quantitative flow cytometry.
Focused on measuring the levels of the CD20 surface antigen, a target for rituximab therapy.

Main Results:

Successfully developed a quantitative flow cytometry approach.
Demonstrated the ability to measure CD20 surface antigen levels.

Conclusions:

This quantitative method provides critical data for monitoring antibody-based therapies.
Precise antigen quantitation can inform treatment adjustments and dosing strategies.