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Updated: Jul 3, 2026
07:06
Principles of Site-Specific Recombinase (SSR) Technology
Published on: May 29, 2008
In vivo site-specific recombination using the beta-rec/six system
Pilar Servert1, Vicente Díaz, Daniel Lucas
1Departamento de Inmunología y Oncología, Campus Universidad Autónoma de Madrid, Madrid, Spain.
Biotechniques
|July 10, 2008
Summary
The beta-serine recombinase (beta-rec) system enables precise gene targeting in mammalian cells. This study demonstrates its functionality for in vivo genome modifications using transgenic mice.
Area of Science:
- Molecular Biology
- Genetics
- Gene Editing
Background:
- Prokaryotic beta-serine recombinase (beta-rec) mediates site-specific recombination.
- The beta-rec/six system offers controlled recombination for complex genetic manipulations.
Purpose of the Study:
- To explore the beta-rec/six system for selective genome-targeted modifications.
- To assess the in vivo functionality of beta-rec for gene targeting.
Main Methods:
- Generated transgenic mouse lines (Tgbeta) expressing beta-rec under the Lck promoter.
- Developed transgenic mouse lines (Tgrec and KOsix) with specific target sequences.
- Analyzed beta-rec activity and its effects on T cell development and genome targeting.
Main Results:
- Demonstrated beta-rec activity in transgenic mice without adverse effects on T cell development.
- Confirmed the functionality of the beta-rec/six system for in vivo gene targeting.
Conclusions:
- The beta-rec/six site-specific recombination system is effective for in vivo gene targeting applications.
- This system provides a powerful tool for precise genome modifications in mammalian systems.
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