Direct electrogenerated chemiluminescence detection in high-performance liquid chromatography for determination of
Yonghua Sun1, Zhujun Zhang, Zhijun Xi
1College of Chemistry and Materials Science, Shaanxi Normal University, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Xi'an 710062, China.
Abstract:
Ofloxacin (OFLX) exhibited strong electrogenerated chemiluminescence (ECL) in NaNO(3) solution with a dual-electrode system when constant current was exerted. Based on this observation, a sensitive direct ECL method coupled with high-performance liquid chromatography (HPLC) separation was developed for determination of OFLX in human serum. Factors affected the ECL emission were investigated. Under the optimal conditions, the ECL intensity has a linear relationship with the concentration of OFLX in the range of 1.0x10(-8) to 4.0x10(-6) g mL(-1) and the detection limit was 4x10(-9) g mL(-1) (S/N=3). The proposed method was sensitive, simple and convenient to operate.
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