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Updated: Jul 3, 2026
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In Situ Hybridization for the Precise Localization of Transcripts in Plants
Published on: November 23, 2011
In situ Hybridization
1Department of Allergy and Clinical Immunology, National Heart and Lung Institute, Imperial College, South Kensington, London, UK.
Methods in Molecular Medicine
|July 10, 2008
Summary
In situ hybridization detects specific DNA or RNA sequences within cells and tissues. This sensitive technique localizes gene expression, viral infections, and maps gene sequences, with applications in archival and frozen samples.
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Background:
- Hybridization involves forming nucleic acid hybrids (DNA:DNA, DNA:RNA, RNA:RNA).
- In situ hybridization (ISH) is a sensitive method for detecting and localizing specific nucleic acid sequences within cells or tissues.
- ISH is applicable to various sample types, including preserved cells, tissue sections, and chromosome preparations.
Purpose of the Study:
- To detail the principles and applications of in situ hybridization.
- To highlight the advantages and major steps of the in situ hybridization technique.
- To provide a comprehensive overview of in situ hybridization for scientific research.
Main Methods:
- Probe preparation and labeling.
- Tissue fixation and permeabilization.
- Hybridization and signal detection.
Main Results:
- In situ hybridization enables the localization of viral infections.
- It identifies sites of gene expression and analyzes mRNA transcription and tissue distribution.
- ISH can map gene sequences to specific chromosomal locations.
Conclusions:
- In situ hybridization is a versatile and sensitive technique for nucleic acid detection in situ.
- Its applications span diagnostics, gene mapping, and expression analysis.
- The method can be combined with immunohistochemistry for multi-analyte detection and is applicable to diverse sample types.
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02:31In-situ Hybridization
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
02:07FISH - Fluorescent In-situ Hybridization
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...