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Related Experiment Video

Updated: Jul 3, 2026

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

Double emulsion templated monodisperse phospholipid vesicles.

Ho Cheung Shum1, Daeyeon Lee, Insun Yoon

  • 1School of Engineering and Applied Sciences and Department of Physics, Harvard University, Cambridge, Massachusetts 02138, USA.

Langmuir : the ACS Journal of Surfaces and Colloids
|July 11, 2008
PubMed
Summary

Researchers developed a new method for creating uniform phospholipid vesicles using double emulsion templates. This technique ensures high encapsulation efficiency and works with various phospholipids.

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Area of Science:

  • Biomaterials Science
  • Microfluidics
  • Lipid Nanotechnology

Background:

  • Phospholipid vesicles are crucial for drug delivery and biomimetic studies.
  • Current fabrication methods often struggle with uniformity and encapsulation efficiency.
  • Controlled templating offers a promising route to overcome these limitations.

Purpose of the Study:

  • To develop a novel, reproducible method for fabricating monodisperse phospholipid vesicles.
  • To achieve high encapsulation efficiency in the resulting vesicles.
  • To demonstrate the versatility of the technique across different phospholipid compositions.

Main Methods:

  • Utilizing glass-capillary microfluidics to generate highly monodisperse double emulsion templates.
  • Employing a solvent removal process to transition from double emulsion templates to phospholipid vesicles.

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Last Updated: Jul 3, 2026

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting
08:35

Lipid Bilayer Vesicle Generation Using Microfluidic Jetting

Published on: February 21, 2014

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays
11:03

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Published on: March 9, 2021

  • Characterizing vesicle size, shape, and uniformity post-fabrication.

    • Main Results:

    • Successfully fabricated monodisperse phospholipid vesicles with controlled sizes.
    • Demonstrated high encapsulation efficiency within the phospholipid vesicles.
    • Confirmed that the uniformity of the double emulsion templates is preserved in the final vesicles.
    • Validated the technique's applicability to a broad spectrum of phospholipids.

    Conclusions:

    • The developed microfluidic approach provides a robust platform for producing uniform phospholipid vesicles.
    • This method offers a significant advancement in vesicle fabrication, enabling high encapsulation efficiency.
    • The technique's versatility makes it suitable for diverse applications in lipid-based nanotechnology and drug delivery.