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Updated: Jul 3, 2026
Measurement of Strial Blood Flow in Mouse Cochlea Utilizing an Open Vessel-Window and Intravital Fluorescence Microscopy
Published on: September 21, 2021
Effect of inner ear blood flow changes in Ménière's model mice
Masaya Takumida1, Nana Akagi, Matti Anniko
1Department of Otolaryngology, Hiroshima University Faculty of Medicine, 1-2-3 Kasumicho, Minamiku, Hiroshima, Japan. masati@hiroshima-u.ac.jp
Conclusions:
The endolymphatic sac (ES) is important for inner ear fluid homeostasis. A dysfunctional ES can cause vertigo attacks following additional stress such as a sudden change in endolymphatic volume and/or pressure, or restricted inner ear blood flow.
Objective:
The purpose of this study was to elucidate the mechanism of vertigo attacks in Ménière's disease.
Materials And Methods:
Adult CBA/J mice were given an intratympanic injection of lipopolysaccharide and an intraperitoneal injection of aldosterone. These 'model' animals had epinephrine or sodium nitroprusside (SNP) instilled into the middle ear cavity. Cochleae, vestibules, and endolymphatic sacs were studied morphologically by light microscopy.
Results:
The injection of epinephrine into the model animals reduced the endolymphatic hydrops in the cochlea, but also produced mild hydrops in the vestibule, which was never observed in untreated (control) animals. The ES did not react to epinephrine in the normal way. Injection of SNP did not cause any changes.
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