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Close-to-native ultrastructural preservation by high pressure freezing

Dimitri Vanhecke1, Werner Graber, Daniel Studer

  • 1Institute of Anatomy, University of Bern, 3000 Bern 9, Switzerland.

Methods in Cell Biology
|July 12, 2008
PubMed
Summary

High pressure freezing enables vitrification of biological samples for transmission electron microscopy (TEM), preserving ultrastructure better than chemical fixation. This method is crucial for observing life science specimens closer to their living state.

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