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Updated: Jul 3, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
Published on: July 8, 2025
Synthesis and evaluation of the luciferase-oligodeoxynucleotide for the sequence-selective detection of nucleic acids
Fumi Nagatsugi1, Ryusuke Nakahara, Kaori Inoue
1Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, Japan.
Abstract:
A new method for the synthesis of ODN-luciferase conjugate was investigated as a signal-amplifying sensor of the target nucleic acids. The conjugation of the luciferase was successfully achieved between the cysteine residue and the 2-amino-6-vinylpurine nucleoside of the ODN probe without significant inactivation of luciferase. The ODN-luciferase conjugate modified with PEG retained the luciferase activity and selectivity during the hybridization with the target ODN.
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