Related Experiment Video
Updated: Jul 3, 2026
Differentiated Mouse Adipocytes in Primary Culture: A Model of Insulin Resistance
Published on: February 17, 2023
[Establishing and identifying insulin resistance 3T3-L1 adipocytes cell model]
Xiao-Nong Guo1, Ju-Tian Yang, Feng Niu
1Life Science and Engineering Institute of Northwest University for Nationalities, Lanzhou 730030, China. gxnwww@126.com
Objective:
To establish insulin-resistant 3T3-L1 adipocytes cell model.
Methods:
3T3-L1 preadipocytes were cultured and induced to differentation. We established insulin resistant 3T3-L1 induced by dexamethasone and determined the change of glucose concentration in cell culture.
Results:
In high glucose DMEM culture media, the glusose concentration significantly decreased. Achieved the peak of insulin-resisitant at the 96th hour induced by dexamethasone, and at this time compared with the blank the mRNA expression of Resistin of the model step up about three times.
Conclusion:
3T3-L1 adipocyte exposed to 1 micromol/L dexamethasone for 24 hours are able to induce a state of insulin resistance which can be maintained for 216 hours.
