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Updated: Jul 3, 2026

A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
Dehydroascorbate and glucose are taken up into Arabidopsis thaliana cell cultures by two distinct mechanisms
N Horemans1, A Szarka, M De Bock
1University of Antwerp, Department of Biology, Plant Physiology, Groenenborgerlaan 171, B-2020 Antwerp, Belgium. nele.horemans@ua.ac.be
Abstract:
The possible involvement of glucose (Glc) carriers in the uptake of vitamin C in plant cells is still a matter of debate. For the first time, it was shown here that plant cells exclusively take up the oxidised dehydroascorbate (DHA) form. DHA uptake is not affected by 6-bromo-6-deoxy-ascorbate, an ascorbate (ASC) analogue, specifically demonstrating ASC uptake in animal cells. There is no competition between Glc and DHA uptake. Moreover, DHA and Glc carriers respond in the opposite manner to different inhibitors (cytochalasin B, phloretin and genistein). In conclusion, the plant plasma membrane DHA carrier is distinct from the plant Glc transporters.
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