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Updated: Jul 3, 2026

Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype
Published on: March 18, 2020
Delivery of immunotherapy with peptide-pulsed blood in macaques
Robert De Rose1, Caroline S Fernandez, Liyen Loh
1Department of Microbiology and Immunology, University of Melbourne, 3010, Australia.
Abstract:
Simple and effective delivery methods for cellular immunotherapies are needed. We assessed ex vivo pulsing of overlapping SIV Gag 15mer peptides onto either whole blood or PBMC in 15 randomly assigned SIV-infected macaques. Both delivery methods were safe and immunogenic, stimulating high levels of broad and polyfunctional Gag-specific CD4 and CD8 T cells. Delivery of overlapping Gag peptides via either whole blood or PBMC is suitable for clinical evaluation.

