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Sequestration of basic fibroblast growth factor in the primate retinal interphotoreceptor matrix
G S Hageman1, M A Kirchoff-Rempe, G P Lewis
1Bethesda Eye Institute, Department of Ophthalmology, St. Louis University School of Medicine, MO 63110.
Summary
Basic fibroblast growth factor (bFGF) was found in the interphotoreceptor matrix (IPM) of the retina. This suggests bFGF may play a role in regulating retinal cell functions.
Area of Science:
- Ophthalmology
- Cell Biology
- Biochemistry
Background:
- The interphotoreceptor matrix (IPM) is an extracellular space in the retina containing insoluble glycoconjugates.
- The function of many IPM components remains unclear, though some may aid retinal adhesion or photoreceptor survival.
Purpose of the Study:
- To identify constituents of the IPM and investigate their potential roles in retinal function.
- To determine if growth factors are present within the IPM.
Main Methods:
- Immunohistochemistry using antibodies against basic fibroblast growth factor (bFGF).
- Western blotting of purified IPM components and bFGF.
- Analysis of primate retinal sections.
Main Results:
- Basic fibroblast growth factor (bFGF) was identified within the aqueous-insoluble fraction of the IPM in primate retinas.
- Antibodies against bFGF labeled IPM sections and bound to purified insoluble IPM preparations.
- Western blots confirmed the presence of bFGF (16.5-17.5 kDa) in insoluble IPM, not soluble components.
Conclusions:
- Basic fibroblast growth factor (bFGF) is sequestered within the IPM, likely bound to insoluble matrix components.
- The localization of bFGF near photoreceptors, retinal pigmented epithelium, and Mueller's glia suggests it may regulate these cells in vivo.