Related Experiment Video
Updated: Jul 3, 2026
Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
Published on: March 14, 2019
The kinetics of RCC1 inclusion body formation in Escherichia Coli
A M Tsai1, M J Betenbaugh, J Shiloach
1Biotechnology Unit, NIDDK, NIH, Bethesda, Maryland 20892.
Abstract:
The Regulator of Chromosome Condensation protein (RCC1) is located in both the soluble and inclusion body (IB) fractions of the whole cell lysate when expressed in Escherichia coli BL21 (pLysS) at temperatures below 30 degrees C. When bacterial growth was carried out at 20 degrees C, the majority of the RCC1 remained soluble up to 5.5 h postinduction, When the temperature was raised to 25 degrees C, RCC1 IB was dominant by 1.5 h postinduction. The shift in RCC1 IB formation with temperature suggests that in addition to increased translation rates, folding and aggregation processes may contribute to RCC1 IB formation at higher temperatures. (c) 1995 John Wiley & Sons, Inc.
