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Related Concept Videos

Selectins01:25

Selectins

Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...
Protein Glycosylation01:25

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...

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Related Experiment Video

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Glycan Profiling of Plant Cell Wall Polymers using Microarrays
12:30

Glycan Profiling of Plant Cell Wall Polymers using Microarrays

Published on: December 17, 2012

Lectin microarrays identify cell-specific and functionally significant cell surface glycan markers.

Sheng-Ce Tao1, Yu Li, Jiangbing Zhou

  • 1Department of Pharmacology and Molecular Sciences, Johns Hopkins University, Baltimore, MD 21205, USA.

Glycobiology
|July 16, 2008
PubMed
Summary

This study introduces a high-throughput lectin microarray to analyze cell surface glycan signatures. This tool aids in understanding cell development, pathogen interactions, and identifying cancer biomarkers.

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Published on: September 7, 2019

Area of Science:

  • Biochemistry
  • Cell Biology
  • Glycomics

Background:

  • Glycosylation, a complex posttranslational modification, presents challenges for high-throughput analysis due to its diversity.
  • Understanding cell surface glycans is crucial for various cellular processes, including development, differentiation, and disease.
  • Existing methods are often insufficient for comprehensive, high-throughput analysis of glycosylation patterns.

Purpose of the Study:

  • To develop a high-throughput lectin microarray for defining mammalian cell surface glycan signatures.
  • To establish a method for analyzing cell binding and hierarchical organization based on glycan profiles.
  • To demonstrate the utility of glycan signatures in predicting biological and clinical relevance.

Main Methods:

  • Development and application of a high-throughput lectin microarray platform.
  • Analysis of cell surface glycan signatures in 24 mammalian cell lines.
  • Documentation of glycosylation changes during murine immune cell development and differentiation.
  • Application of glycan signatures to predict pathogen tropism and identify cancer stem cell biomarkers.

Main Results:

  • Established a robust method for high-throughput glycan signature analysis using lectin microarrays.
  • Characterized glycan profiles of diverse mammalian cell lines and tracked changes during cell differentiation.
  • Demonstrated the predictive power of glycan signatures for pathogen tropism (mannose-dependent).
  • Identified novel lectin biomarkers for cancer stem-like cells in a murine model.

Conclusions:

  • Lectin microarrays provide an effective high-throughput tool for analyzing complex cell surface glycosylation.
  • Glycan signatures are valuable for understanding cell development, differentiation, and cell-cell communication.
  • This technology has significant potential for pathogen-host recognition studies and biomarker discovery in cancer.