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Updated: Jul 3, 2026
09:07
Murine Model of Epicutaneously-Induced Immunomodulation
Published on: June 24, 2025
Modification of native collagen reduces antigenicity but preserves cell compatibility
J Hardin-Young1, R M Carr, G J Downing
1Organogenesis Inc., 150 Dan Road, Canton, Massachusetts 02021.
Biotechnology and Bioengineering
|March 20, 1996
Summary
Porcine intestinal collagen (ICL) processing reduces its antigenicity. Modified ICL, specifically PA/EDC-ICL, elicits a significantly lower humoral immune response in both rabbit and canine models, indicating its potential for improved vascular prostheses.
Area of Science:
- Biomaterials Science
- Immunology
- Vascular Surgery
Background:
- Porcine intestinal collagen (ICL) is a key component in biosynthetic vascular prostheses.
- The processing of ICL involves mechanical cleaning, disinfection (peracetic acid - PA), and crosslinking (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride - EDC).
- Understanding the immunogenicity of processed ICL is crucial for developing effective vascular grafts.
Purpose of the Study:
- To evaluate the impact of PA and EDC treatments on the humoral immune response to porcine intestinal collagen (ICL).
- To assess the antigenicity of non-crosslinked ICL (NC-ICL), PA-ICL, and PA/EDC-ICL in preclinical models.
- To determine the immunogenicity of PA/EDC-ICL vascular grafts in a canine model.
Main Methods:
- Rabbits were immunized with NC-ICL, PA-ICL, and PA/EDC-ICL to determine antibody titers.
- Proteins were extracted and separated using SDS-PAGE, and immunoblots were performed.
- Canine subjects received PA/EDC-ICL vascular grafts, and serum was analyzed for antibodies to ICL and type I collagen at various time points.
Main Results:
- PA and EDC treatments reduced the number of extractable proteins from ICL.
- Anti-NC-ICL antibodies recognized multiple proteins in NC-ICL, but not in PA-ICL or PA/EDC-ICL.
- Rabbits immunized with NC-ICL showed higher antibody titers compared to those immunized with PA-ICL or PA/EDC-ICL, indicating NC-ICL is more antigenic.
- In canines, no serum antibodies to ICL proteins or type I collagen were detected at 4, 8, or 12 weeks post-implantation of PA/EDC-ICL grafts.
Conclusions:
- The processing of porcine intestinal collagen with peracetic acid and EDC significantly reduces its immunogenicity.
- PA/EDC-ICL is less antigenic than non-processed or PA-only treated ICL.
- PA/EDC-ICL fabricated vascular grafts demonstrate a reduced humoral immune response in a canine model, suggesting improved biocompatibility for vascular applications.
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