Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 3, 2026

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

Published on: November 30, 2022

Functional characterization of Lmo2-Cre transgenic zebrafish.

Lei Wang¹, Yong Zhang, Ting Zhou

¹Laboratory of Development and Diseases, Institute of Health Sciences, Chinese Academy of Sciences and Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Developmental Dynamics : an Official Publication of the American Association of Anatomists

|July 16, 2008

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

TRAIL and Celastrol Combinational Treatment Suppresses Proliferation, Migration, and Invasion of Human Glioblastoma Cells via Targeting Wnt/β-catenin Signaling Pathway.

Chinese journal of integrative medicine·2023

Same author

LWJ-M30, a conjugate of DM1 and B6, for the targeted therapy of colorectal cancer with improved therapeutic effects.

RSC advances·2023

Same author

XHL11, a novel selective EGFR inhibitor, overcomes EGFR<sup>T790M</sup>-mediated resistance in non-small cell lung cancer.

European journal of pharmacology·2021

Same author

Poor preoperative sleep quality is a risk factor for severe postoperative pain after breast cancer surgery: A prospective cohort study.

Medicine·2019

Same author

The histone demethylase Jmjd3 regulates zebrafish myeloid development by promoting spi1 expression.

Biochimica et biophysica acta. Gene regulatory mechanisms·2018

Same author

The effect of intraperitoneal chemotherapy on early pain hyperalgesia in patients following elective laparoscopic transabdominal resection of rectal cancer.

Oncotarget·2017

A new transgenic zebrafish line, Tg(zlmo2:Cre), enables precise genetic manipulation in hematopoietic progenitors and vascular endothelial cells. This tool is crucial for studying blood development and related diseases.

Area of Science:

Genetics
Developmental Biology
Zebrafish Models

Background:

The Cre/loxP system is vital for genomic manipulation, enabling conditional gene targeting and lineage tracing in transgenic organisms.
Stable Cre expression in specific zebrafish tissues is lacking, hindering detailed genetic analysis.
Understanding gene function in early development requires precise genetic tools.

Purpose of the Study:

To establish and characterize a stable transgenic zebrafish line with Cre recombinase expression driven by the lmo2 promoter.
To validate the functional activity of the Tg(zlmo2:Cre) line for genetic studies.
To provide a novel tool for investigating hematopoietic and vascular development in zebrafish.

Main Methods:

Generation of a stable transgenic zebrafish line, Tg(zlmo2:Cre), utilizing the lmo2 promoter.

More Related Videos

Zebrafish Model of Neuroblastoma Metastasis

Zebrafish Model of Neuroblastoma Metastasis

Published on: March 14, 2021

In Vivo Modeling of the Morbid Human Genome using Danio rerio

In Vivo Modeling of the Morbid Human Genome using Danio rerio

Published on: August 24, 2013

Related Experiment Videos

Last Updated: Jul 3, 2026

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

Published on: November 30, 2022

Zebrafish Model of Neuroblastoma Metastasis

Zebrafish Model of Neuroblastoma Metastasis

Published on: March 14, 2021

In Vivo Modeling of the Morbid Human Genome using Danio rerio

In Vivo Modeling of the Morbid Human Genome using Danio rerio

Published on: August 24, 2013

Analysis of Cre expression patterns during zebrafish embryogenesis.

Crossing Tg(zlmo2:Cre) with a Cre-reporter line (Tg(zlmo2:loxP-DsRed-loxP-EGFP)) to assess recombination activity.

Main Results:

The Tg(zlmo2:Cre) line exhibits stable and specific Cre expression in primitive hematopoietic progenitors and vascular endothelial cells.
The observed Cre expression pattern mirrors the endogenous lmo2 expression during embryogenesis.
Robust recombination was confirmed in hematopoietic progenitors and vascular endothelial cells upon crossing with the reporter line.

Conclusions:

The Tg(zlmo2:Cre) transgenic zebrafish line is successfully established and functionally validated.
This line provides a powerful and specific tool for conditional gene manipulation in hematopoietic and vascular systems.
It will significantly advance research into hematopoietic development and associated diseases in zebrafish models.