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Updated: Jul 3, 2026
12:16
Robust 3D DNA FISH Using Directly Labeled Probes
Published on: August 15, 2013
FISH for mapping single copy genes.
Terje Raudsepp1, Bhanu P Chowdhary
1Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX, USA.
Methods in Molecular Biology (Clifton, N.J.)
|July 17, 2008
Summary
Fluorescent in-situ hybridization (FISH) is a key genomic technique for gene localization. This guide details essential FISH procedures, from target preparation to imaging, for researchers.
Area of Science:
- Genomics
- Molecular Biology
- Cytogenetics
Background:
- Fluorescent in-situ hybridization (FISH) has been a cornerstone technique in genomics for over 20 years.
- FISH enables direct localization, orientation, and ordering of genes within diverse species' genomes.
- Despite technological advancements, the fundamental FISH protocols remain consistent.
Purpose of the Study:
- To provide a comprehensive, stepwise overview of core Fluorescent in-situ hybridization (FISH) procedures.
- To consolidate essential information on target preparation, probe labeling, hybridization, signal detection, and imaging.
- To serve as a practical guide for researchers utilizing FISH technology.
Main Methods:
- Detailed explanation of target preparation, including metaphase/interphase chromosomes and DNA fibers.
- Stepwise description of probe labeling strategies and techniques.
- Comprehensive guide to in-situ hybridization, signal detection, and advanced imaging methods.
Main Results:
- A clear, sequential methodology for performing FISH experiments.
- Insights into optimizing each step of the FISH protocol for reliable results.
- Emphasis on the enduring core procedures despite evolving technologies.
Conclusions:
- Fluorescent in-situ hybridization (FISH) remains a vital and standardized technique in genomics.
- Mastery of core FISH procedures is essential for accurate gene mapping and genomic analysis.
- This overview facilitates consistent and effective application of FISH across various research settings.
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