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Updated: Jul 3, 2026
07:44
Pancreatic Islet Isolation and Purification from Lewis Rats Using Enzymatic Digestion and Density-Gradient Separation
Published on: April 3, 2026
[An economic and effective method for islet cell isolation from SD rats].
Jianbo Zhang1, Qian Yang, Jie Chen
1People's Hospital, Chengdu Sichuan, 610072, P.R. China.
Summary
This study presents an economical rat islet isolation protocol using compound sodium chloride injection and low-concentration collagenase V. This method improves islet recovery and function, proving effective for creating animal models for islet transplantation.
Area of Science:
- Endocrinology
- Surgical Research
- Biomedical Engineering
Context:
- Pancreatic islet isolation is crucial for diabetes research and transplantation.
- Existing methods can be costly and may impact islet viability.
- Developing efficient and cost-effective isolation techniques is essential.
Purpose:
- To establish an economical and effective method for rat islet isolation.
- To validate a laboratory protocol for generating an animal model for clinical islet transplantation.
Summary:
- Rat islets were isolated using intraductal infusion of compound sodium chloride injection (CSCI) and digested with low-concentration collagenase V (0.5 mg/mL).
- Purification employed discontinuous density gradient centrifugation with Ficoll 400.
- Islet yield, purity (90.2%), viability (81.6%), and insulin secretion function were assessed.
Impact:
- The developed method significantly reduces experimental costs.
- It demonstrates a beneficial effect on islet recovery rates and preserves endocrine function.
- This protocol provides a reliable animal model for islet transplantation studies.
