Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis pathway,...
Nucleic Acids02:43

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
Nucleic acids02:43

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TREADS: tyre nanoparticles produced using a bench-top tyre wear simulator.

Environmental science. Nano·2026
Same author

iMUT-seq mapping of DSB-induced mutations with high sensitivity at single-nucleotide resolution.

Nature protocols·2026
Same author

Increased mRNA translation delays tumour initiation and exposes a therapeutic vulnerability in lung cancer.

Molecular cancer·2026
Same author

The yeast phosphofructokinase β-subunit has RNA unwinding activity and modulates cell cycle progression.

Nucleic acids research·2026
Same author

Functional characterisation of tumour suppressor PDCD4 reveals previously undisclosed role in the control of cell adhesion.

Nucleic acids research·2026
Same author

CBL ubiquitin ligase targets translation as a degrader E3.

Chemical science·2026

Related Experiment Video

Updated: Jul 3, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Polypyrimidine-tract-binding protein: a multifunctional RNA-binding protein.

Kirsty Sawicka1, Martin Bushell, Keith A Spriggs

  • 1Centre for Biomolecular Sciences, School of Pharmacy, University of Nottingham, University Park, Nottingham, UK.

Biochemical Society Transactions
|July 18, 2008
PubMed
Summary

Polypyrimidine-tract-binding protein (PTB) regulates diverse cellular processes, including mRNA stability and translation initiation. This review explores how PTB controls these functions through localization and protein interactions.

More Related Videos

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

Published on: December 2, 2009

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

Related Experiment Videos

Last Updated: Jul 3, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

Published on: December 2, 2009

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

Area of Science:

  • Molecular Biology
  • Cell Biology
  • RNA Biology

Background:

  • Polypyrimidine-tract-binding protein (PTB) is a ubiquitous RNA-binding protein.
  • Initially identified for its role in splicing, PTB is now recognized for its involvement in polyadenylation, mRNA stability, and translation initiation.

Purpose of the Study:

  • To review the multifaceted roles of PTB in regulating cellular processes.
  • To elucidate how PTB's cellular localization and protein interactions dictate its function.

Main Methods:

  • Literature review of studies on PTB function.
  • Analysis of PTB's role in splicing, polyadenylation, mRNA stability, and translation initiation.
  • Examination of PTB's interaction with other proteins and its subcellular localization.

Main Results:

  • PTB acts as both a splicing suppressor and a translation activator.
  • PTB's function is modulated by its controlled nucleocytoplasmic shuttling and interactions with other trans-acting factors.
  • PTB is crucial for internal ribosome entry site (IRES)-mediated translation initiation.

Conclusions:

  • PTB plays critical, context-dependent roles in gene expression regulation.
  • Understanding PTB's regulatory mechanisms provides insights into cellular growth and death pathways.
  • PTB's involvement in IRES-mediated translation highlights its importance in viral and cellular protein synthesis.