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Related Experiment Video

Updated: Jul 3, 2026

Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation

Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation

Published on: September 14, 2011

Evidence for changes in nuclear permeability during different physiological states.

¹Department of Anatomical Sciences, University of Florida, Gainesville, Florida.

|January 1, 1971

Summary

Well-fed amoebae nuclei accumulate colloidal gold faster than starved cells, indicating starvation reduces nuclear envelope permeability. This suggests nuclear pores regulate cellular activity by altering their functional properties.

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Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria

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Biophysical Assays to Probe the Mechanical Properties of the Interphase Cell Nucleus: Substrate Strain Application and Microneedle Manipulation

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Published on: September 14, 2011

Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria

Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria

Published on: September 7, 2012

Area of Science:

Cell Biology
Molecular Biology
Biophysics

Background:

The nuclear envelope regulates transport between the cytoplasm and nucleus.
Nuclear pores are critical for this transport, but their dynamic regulation is not fully understood.
Starvation is known to induce cellular changes, potentially affecting nuclear transport.

Purpose of the Study:

To investigate the effect of starvation on nuclear envelope permeability in amoebae.
To determine the mechanism by which starvation impacts nuclear transport.
To explore the role of nuclear pores in regulating cellular activity under different nutritional conditions.

Main Methods:

Utilized colloidal gold particles as tracers for nuclear uptake in amoebae.
Compared the rate of colloidal gold accumulation in the nuclei of well-fed versus starved amoebae (5 and 9 days).
Analyzed changes in nuclear envelope permeability and nuclear pore function.

Main Results:

Nuclei of well-fed amoebae demonstrated significantly higher colloidal gold accumulation rates compared to starved cells.
Starvation led to a decrease in nuclear envelope permeability.
Evidence suggests altered functional properties of nuclear pores, not changes in pore size or number, caused this permeability decrease.

Conclusions:

Nuclear envelope permeability decreases during starvation in amoebae.
The functional properties of nuclear pores are dynamically regulated and are responsible for changes in permeability.
These findings support the model where nuclear pores play a key role in regulating cellular activity and adaptation.