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Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
A procedure for rapid isolation and analysis of cauliflower mosaic virus DNA
1Department of Plant Pathology, University of California, Davis, California 95616, USA.
Virology
|October 15, 1980
Abstract:
A simple and rapid procedure has been developed for the small-scale isolation of crude preparations of cauliflower mosaic virus DNA. The preparations are relatively free of contaminating cellular DNA and are sufficiently pure to be digested with restriction enzymes. The method enables plants infected with single-lesion isolates of mutagenized viral DNA to be rapidly screened for particular restriction endonuclease sites.
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