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In Vitro Transcribed RNA-based Luciferase Reporter Assay to Study Translation Regulation in Poxvirus-infected Cells
Published on: May 1, 2019
In vitro translation of tobacco etch virus RNA
1Department of Biological Sciences, Wayne State University, Detroit, Michigan, USA.
Virology
|October 15, 1980
Abstract:
The wheat-embryo-derived cell-free system was optimized for translation of tobacco etch virus (TEV) RNA. When examined by polyacrylamide gel electrophoresis, the product of the TEV RNA stimulated system proved to be a single protein with a molecular weight of 40,000. This finding is different from results obtained when TEV RNA is used as a template in the rabbit reticulocyte lysate in vitro protein-synthesizing system.
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