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Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
Published on: November 1, 2011
The genome-linked protein of cowpea mosaic virus is coded by RNA from the bottom component
J Stanley1, R Goldbach, A Van Kammen
1Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands.
Abstract:
It has recently been shown that RNA from the bottom component of cowpea mosaic virus is capable of self-replication in cowpea mesophyll protoplasts, providing a tool for the investigation of the contribution of each RNA strand toward viral multiplication. Evidence is presented here, using this system, to show that the genome-linked protein is coded by the RNA from the bottom component. As the replicase function is coded by this RNA, the results remain consistent with the involvement of the genome-linked protein in viral RNA replication.
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