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Succinic acid production from continuous fermentation process using Mannheimia succiniciproducens LPK7
In Jae Oh1, Hye Won Lee, Chul Hwan Park
1Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Korea.
Journal of Microbiology and Biotechnology
|July 18, 2008
Summary
Mannheimia succiniciproducens LPK7 was engineered for enhanced succinic acid production. Optimal continuous fermentation conditions achieved high succinic acid yield and productivity, demonstrating industrial applicability.
Area of Science:
- Microbial biotechnology
- Metabolic engineering
- Industrial microbiology
Background:
- Succinic acid is a valuable platform chemical with growing industrial demand.
- Mannheimia succiniciproducens LPK7 is a metabolically engineered strain designed to improve succinic acid yield and reduce by-product formation.
- Optimizing fermentation conditions is crucial for enhancing succinic acid productivity and industrial viability.
Purpose of the Study:
- To optimize anaerobic continuous fermentation conditions for Mannheimia succiniciproducens LPK7.
- To evaluate the industrial applicability of the engineered strain for succinic acid production.
- To analyze metabolic flux distribution to understand quantitative balances.
Main Methods:
- Anaerobic continuous fermentation of Mannheimia succiniciproducens LPK7 at varying glucose feed concentrations and dilution rates.
- Strain engineering involving knock-outs of lahA, pflB, and pta-ackA genes.
- Metabolic flux analysis using a model of glycolysis and the pentose phosphate pathway.
Main Results:
- Achieved a succinic acid yield of 0.38 mol/mol and productivity of 1.77 g/l/h.
- Optimal conditions identified as 18.0 g/l glucose feed concentration and 0.2 h⁻¹ dilution rate.
- Comparable succinic acid production was observed in batch cultures.
Conclusions:
- The identified optimal conditions are suitable for the industrial-scale continuous production of succinic acid.
- Metabolic flux analysis provides insights into the quantitative metabolic balance during fermentation.
- The engineered M. succiniciproducens LPK7 strain shows promise for efficient succinic acid manufacturing.
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