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Preparation and Immunostaining of Myelinating Organotypic Cerebellar Slice Cultures
Published on: March 20, 2019
Axonal cell-adhesion molecule L1 in CNS myelination
G Barbin1, M S Aigrot, P Charles
1Biologie des Interactions Neurones/Glie, UPMC, INSERM U-495, Hôpital de la Salpêtrière, Paris Cedex 13.
Neuron Glia Biology
|July 19, 2008
Summary
Axonally expressed L1 cell adhesion molecule is crucial for initiating central nervous system (CNS) myelination. Blocking L1 function inhibits myelination, suggesting its role in early oligodendrocyte development.
Area of Science:
- Neuroscience
- Cell Biology
- Molecular Biology
Background:
- Adhesion molecules on axons are potential mediators of myelinating cell-axon interactions.
- L1 cell-adhesion molecule (a neural cell adhesion molecule) is vital for neuronal development and peripheral nervous system (PNS) myelination.
Purpose of the Study:
- To investigate the role of axonally expressed L1 in central nervous system (CNS) myelination.
- To elucidate the molecular mechanisms by which L1 influences CNS myelination.
Main Methods:
- Co-culture of murine oligodendrocytes and neurons.
- Immunohistochemistry to detect L1 expression patterns.
- Inhibition of L1 function using anti-L1 antibodies and L1-Fc fusion proteins.
- Analysis of mitogen-activated protein (MAP) kinase pathway activation in oligodendrocytes.
Main Results:
- L1 is expressed on neurites before myelination and downregulated during myelination.
- Inhibition of L1 prevents myelination in vitro.
- L1-Fc fusion protein inhibits lysophosphatidic acid-induced MAP-kinase pathway activation in oligodendrocytes.
Conclusions:
- Axonally expressed L1 is essential for the initiation of CNS myelination.
- L1 may mediate CNS myelination through modulation of oligodendroglial phosphoprotein dephosphorylation, potentially involving the MAP-kinase pathway.
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