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Double-stranded ribonucleic acid from carnation cryptic virus.

V Lisa1, G Boccardo, R G Milne

  • 1Istituto di Fitovirologia applicata del CNR, Via O. Vigliani 104, 10185 Torino, Italy.

Virology
|December 1, 1981
PubMed
Summary
This summary is machine-generated.

Carnation cryptic virus RNA was identified as double-stranded RNA (dsRNA). Electrophoresis revealed three major RNA segments with a total molecular weight of approximately 4 x 10^6 Daltons.

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Area of Science:

  • Plant Virology
  • Molecular Biology
  • Nucleic Acid Chemistry

Background:

  • Carnation cryptic virus (CCV) is a plant pathogen.
  • The nature of CCV's genetic material was previously uncharacterized.

Purpose of the Study:

  • To identify and characterize the nucleic acid of carnation cryptic virus.
  • To determine the structure and molecular weight of CCV RNA.

Main Methods:

  • Enzymatic assays using RNase and DNase under varying salt concentrations.
  • Immunological detection using dsRNA-specific antibodies.
  • Electron microscopy in nondenaturing conditions.
  • Polyacrylamide gel electrophoresis (PAGE) for RNA separation and molecular weight estimation.

Main Results:

  • CCV nucleic acid demonstrated susceptibility to RNase in low salt and resistance in high salt, characteristic of dsRNA.
  • CCV RNA reacted with dsRNA-specific antiserum.
  • Electron microscopy revealed morphology consistent with dsRNA.
  • PAGE analysis separated CCV RNA into three major and one minor segment, totaling approximately 4 x 10^6 Daltons.

Conclusions:

  • The nucleic acid of carnation cryptic virus is double-stranded RNA (dsRNA).
  • CCV possesses a segmented dsRNA genome with a total molecular weight of approximately 4 x 10^6 Daltons.