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Technique of venous catheterization for sequential blood sampling from the pig
S A Bain1, J Ting, C J Simeonovic
1Division of Clinical Sciences, John Curtin School of Medical Research, Australian National University, Canberra City.
Summary
This study presents a novel surgical technique for implanting catheters in pigs for long-term blood sampling. The method enhances animal welfare and ensures accurate metabolic data by minimizing stress and catheter dislodgement.
Area of Science:
- Veterinary Surgery
- Animal Models
- Physiological Monitoring
Background:
- Accurate long-term metabolic assessment in animal models requires minimizing stress and preventing catheter dislodgement.
- Existing methods for catheter implantation in pigs may compromise animal welfare and data integrity.
Purpose of the Study:
- To describe a refined surgical technique for chronic indwelling catheter implantation in pigs.
- To improve the reliability and longevity of catheters used for sequential blood sampling in swine.
Main Methods:
- A vinyl catheter was implanted into the vena cava via the external jugular vein.
- Catheter retention was secured using a silicone plug, double venous ligation, dorsal midline exit, and a custom-fitted denim vest.
Main Results:
- The described technique demonstrated high success rates for catheter function.
- Catheters maintained functionality for extended periods, with some exceeding 150 days.
Conclusions:
- This surgical approach offers a reliable and minimally invasive method for chronic catheterization in pigs.
- The technique supports accurate long-term metabolic studies by ensuring catheter stability and animal comfort.