Related Experiment Video
Updated: Jul 3, 2026

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
Published on: December 7, 2021
[Isolation and characterization of a phenanthrene-degrading bacterial strain]
Fang-fang Ai1, Yuan-yuan Qu, Ji-ti Zhou
1School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian 116024, China.
Abstract:
A bacterial strain that degrades phenanthrene was isolated from activated sludge of wastewater treatment plant from a chemical corporation. The strain can utilize phenanthrene as sole source of carbon and energy. It was identified as Burkholderia sp. according to morphological and 16S rRNA gene sequence analysis. Phenanthrene (100 mg x L(-1)) was removed 74.1% within 84 h by this strain. It was suggested that the strain possessed the salicylic acid pathway by LC-MS and UV methods. And the strain contains an inductive catechol 2,3-dioxygenase (C230). The kinetic constants Km and Vmax were 10.93 micromol x L(-1) and 106.38 micromol x min(-1), receptively. It indicates that the affinity of the endoenzyme to catechol is great and catechol is a better substrate.
Related Concept Videos
Microbial Bioremediation of Pesticides
Microbial Bioremediation of Plastics
Microbial Bioremediation of Hydrocarbons

