Related Experiment Video
Updated: Jul 3, 2026

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes
Published on: September 27, 2014
Growth cycle of a virus, PBCV-1, that infects Chlorella-like algae
J L Van Etten1, D E Burbank, Y Xia
1Department of Plant Pathology University of Nebraska, Lincoln, Nebraska, 68583, USA.
Abstract:
The growth and purification of milligram quantities of a large double-stranded DNA virus, PBCV-1, which replicates in a Chlorella-like alga is described. The virus had an adsorption rate constant of ca. 5 x 10(-9) ml/min, a latent period of 150 to 180 min, and a burst size of 200 to 350 PFUs when the host was actively growing in the light. The eclipse period was 30 to 50 min shorter than the latent period. PBCV-1 also replicated in dark grown Chlorella but the burst size was reduced ca. 50%. The photosynthetic inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, had no effect on viral replication. Thus viral replication does not require host photosynthesis. Viral infection rapidly inhibited both growth and CO2 fixation of the host Chlorella.
Related Concept Videos
Lytic Cycle of Bacteriophages
Lysogenic Cycle of Bacteriophages
Viral Replication: Lytic Cycle
Retrovirus Life Cycles
What are Viruses?
Viral Replication: Lysogenic Cycle

