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Updated: Jul 3, 2026

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions
Published on: May 27, 2021
Topology of a ternary complex (proparacaine-beta-cyclodextrin-liposome) by STD NMR
Luís Fernando Cabeça1, Sergio Antonio Fernandes, Eneida de Paula
1Institute of Chemistry, UNICAMP, CP 6154, 13083-970 Campinas, SP, Brazil.
Abstract:
The topologies of proparacaine (PPC) in beta-cyclodextrin (beta-CD), PPC in egg phosphatidylcholine (EPC) liposomes and PPC in beta-CD in EPC were investigated using NMR experiments (1D ROESY and saturation transfer difference (STD)). This is the first description of the STD technique applied to PPC-EPC-beta-CD system, revealing that not only PPC was imbedded in EPC bilayer, but beta-CD was also interacting with liposome vesicles. These results are novel and were rationalized as the spontaneous formation of a ternary complex with some beta-CD molecules bound to external liposome vesicles surfaces.
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