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Related Experiment Video

Updated: Jul 3, 2026

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Assembling DNA barcodes. Analytical protocols.

Jeremy R deWaard¹, Natalia V Ivanova, Mehrdad Hajibabaei

¹Department of Integrative Biology, Biodiversity Institute of Ontario, University of Guelph, Guelph, Canada.

Methods in Molecular Biology (Clifton, N.J.)

|July 23, 2008

Summary

This summary is machine-generated.

The Barcode of Life initiative uses DNA sequencing to identify species. Protocols for animal DNA barcoding are detailed, aiding in species discovery and identification.

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Area of Science:

Genomics
Biodiversity Science
Molecular Biology

Background:

The Barcode of Life initiative aims to create a DNA-based identification system for eukaryotic life.
Significant progress has been made in developing DNA barcodes for the animal kingdom.

Purpose of the Study:

To detail protocols for assembling DNA barcode records for animals.
To support large-scale data collection and identify barcode regions for other eukaryotic kingdoms.

Main Methods:

Selection of a target gene region (cytochrome c oxidase I) for animal DNA barcoding.
Validation of the chosen gene region through pilot studies for species discovery and identification.

Main Results:

Pilot studies confirmed the effectiveness of cytochrome c oxidase I for species identification.
Established protocols provide a foundation for large-scale DNA barcode data assembly.

Conclusions:

DNA barcoding, particularly using cytochrome c oxidase I, is effective for animal species discovery and identification.
The developed protocols are applicable to a broader range of eukaryotic life.