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Highly-Multiplexed Tissue Imaging with Raman Dyes
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Tip-enhanced Raman spectroscopy and microscopy on single dye molecules with 15 nm resolution
Jens Steidtner1, Bruno Pettinger
1Fritz-Haber-Institut der Max-Planck-Gesellschaft, Berlin, Germany.
Physical Review Letters
|July 23, 2008
Abstract:
Our recently developed approach of UHV-tip-enhanced Raman spectroscopy permits us to acquire Raman spectra of a few single brilliant cresyl blue (BCB) molecules and even a single one adsorbed on a Au(111) surface. This is substantiated by simultaneously recorded STM images. Furthermore, because of the reduced photobleaching in UHV, the time frame for spectral acquisition is sufficiently extended to allow tip-enhanced Raman imaging of a single BCB molecule with a lateral resolution of 15 nm.

