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Identification of 30-kDa heat shock protein gene in Trichophyton rubrum
Elham Behzadi1, Payam Behzadi, Fatma Sirmatel
1Clinical Microbiology and Infectious Diseases Department, Faculty of Medicine, Abant Izzet Baysal University, Bolu, Turkey.
Abstract:
Small heat shock proteins (sHSPs) are chaperones that are crucial in the heat shock response but also have important non-stress roles within the cell. HSP70 in Trichophyton rubrum is already detected and carefully characterised; however, no study was carried out for HSP30 in this pathogenic fungus. In the present study, T. rubrum was obtained from patients with dermatophytosis and cultured in appropriate conditions. High-molecular-weight DNA was extracted using standard extraction methods. Pairs of 21 nt primers were designed from highly conserved regions of the similar genes in other eukaryotic cells. Mentioned primers were utilised in PCR using isolated genomic DNA and extracted RNA templates of T. rubrum. The PCR fragments were then sequenced and 415 nucleotides of HSP30 in this pathogenic fungus were detected; the open reading frame had 156 nucleotides and was coding 51 amino acids. This gene (called TrHSP30) is registered in GenBank at National Center for Biotechnology Information (NIH, USA) database. Detection of TrHSP30 gene may open the way to determination of its possible role in the pathogenesis of dermatophyte infections due to T. rubrum.
Insights
Researchers identified the HSP30 gene in Trichophyton rubrum, a fungus causing skin infections. This discovery, named TrHSP30, may help understand its role in dermatophytosis pathogenesis.
Area of Science:
- Mycology
- Molecular Biology
- Biochemistry
Background:
- Small heat shock proteins (sHSPs) are vital chaperones involved in cellular stress responses and non-stress functions.
- While HSP70 in Trichophyton rubrum is characterized, HSP30 in this pathogenic fungus remains unstudied.
- Trichophyton rubrum is a common cause of dermatophytosis.
Purpose of the Study:
- To detect and characterize the HSP30 gene in Trichophyton rubrum.
- To provide a foundation for investigating the role of TrHSP30 in fungal pathogenesis.
Main Methods:
- Genomic DNA and RNA were extracted from cultured Trichophyton rubrum isolates from patients.
- Polymerase Chain Reaction (PCR) was performed using primers designed from conserved eukaryotic HSP30 regions.
- PCR fragments were sequenced to identify the HSP30 gene.
Main Results:
- A 415-nucleotide fragment of the HSP30 gene was detected in Trichophyton rubrum.
- The open reading frame (ORF) was 156 nucleotides, encoding 51 amino acids.
- The identified gene, TrHSP30, was registered in the GenBank database.
Conclusions:
- The TrHSP30 gene has been successfully identified and characterized in Trichophyton rubrum.
- This finding provides a molecular tool for future research into the function of TrHSP30.
- Further studies can now explore the specific role of TrHSP30 in the pathogenesis of dermatophytosis.
