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Performance evaluation of ion exchange and affinity chromatography for HbA1c estimation in diabetic patients with
Bushra Moiz1, Mashhooda Rasool Hashmi, Shahmina Sadaf
1Department of Pathology and Microbiology, The Aga Khan University, Karachi, Pakistan. bushra.moiz@aku.edu
Insights
Boronate affinity chromatography is superior for estimating hemoglobin A1c (HbA1c) in diabetic patients with hemoglobin D (HbD). This method minimizes interference, ensuring accurate glycemic control assessment in both heterozygous and homozygous HbD cases.
Area of Science:
- Clinical Chemistry
- Diabetology
- Chromatography
Background:
- Accurate hemoglobin A1c (HbA1c) measurement is crucial for managing diabetes.
- Hemoglobin variants, such as hemoglobin D (HbD), can interfere with standard HbA1c estimation methods.
- Type I and Type II diabetes patients with HbD require specialized diagnostic approaches.
Purpose of the Study:
- To compare the efficacy of ion exchange chromatography and boronate affinity chromatography for HbA1c estimation.
- To evaluate these methods in diabetic patients with hemoglobin D (both homozygous and heterozygous).
Main Methods:
- Evaluation of systems based on ion exchange chromatography.
- Evaluation of systems based on boronate affinity chromatography.
- Comparative performance analysis in patients with homozygous and heterozygous HbD disease.
Main Results:
- Boronate affinity chromatography demonstrated minimal interference from HbD.
- This interference was reduced in both heterozygous and homozygous HbD diabetic patients.
- Ion exchange chromatography showed greater interference from HbD.
Conclusions:
- Boronate affinity chromatography is a reliable method for HbA1c estimation in diabetic patients with HbD.
- This technique aids in the accurate evaluation of glycemic control in this patient population.
- It offers an advantage over ion exchange chromatography for HbA1c measurement in the presence of HbD.
Objectives:
To compare ion exchange and boronate affinity chromatography for HbA(1c) estimation in patients with type I and II diabetes having hemoglobin D.
Design And Methods:
Systems based on ion exchange and boronate affinity chromatography were evaluated and compared for their performance for HbA(1c) estimation in patients with homozygous and heterozygous D disease.
Results:
Boronate affinity chromatography shows least interference by HbD in heterozygous as well as homozygous diabetic patients for HbA(1c) estimation.
Conclusions:
The use of boronate affinity chromatography was found to be helpful in evaluating glycemic control in diabetic subjects with HbD.