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Related Concept Videos

Selectins01:25

Selectins

Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

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Related Experiment Video

Updated: Jul 3, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

SELEX with modified nucleotides.

Anthony D Keefe1, Sharon T Cload

  • 1Archemix Corp., 300 Third Street, Cambridge, MA 02142, USA. keefe@archemix.com

Current Opinion in Chemical Biology
|July 23, 2008
PubMed
Summary
This summary is machine-generated.

Aptamers are novel therapeutics that function like antibodies. Modified nucleotides enhance aptamer stability and target binding, improving their therapeutic potential.

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Last Updated: Jul 3, 2026

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Published on: July 26, 2010

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Drug Discovery

Background:

  • Aptamers are single-stranded oligonucleotides with therapeutic potential, acting similarly to antibodies by inhibiting target proteins.
  • Current research focuses on enhancing aptamer characteristics for improved drug-like properties.

Purpose of the Study:

  • To explore the use of modified nucleotides in aptamer selection for enhanced therapeutic efficacy.
  • To investigate how nucleotide modifications impact aptamer potency, nuclease resistance, and target affinity.

Main Methods:

  • In vitro selection processes utilizing oligonucleotide libraries with modified nucleotides.
  • Characterization of modified aptamers for stability, nuclease resistance, and target binding affinity.

Main Results:

  • Modified nucleotides significantly enhance aptamer nuclease resistance.
  • Increased target recognition functionality and more stable aptamer structures were observed with modifications.
  • Enhanced aptamer potency was achieved through strategic nucleotide modifications.

Conclusions:

  • Oligonucleotide libraries with modified nucleotides are crucial for developing advanced aptamer therapeutics.
  • Nucleotide modifications offer a viable strategy to improve aptamer drug-like properties, increasing their therapeutic applicability.