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Updated: Jul 3, 2026
Phosphopeptide Analysis of Rodent Epididymal Spermatozoa
Published on: December 30, 2014
Characterizing mouse male germ cell-specific actin capping protein alpha3 (CPalpha3): dynamic patterns of expression
Keizo Tokuhiro1, Yasushi Miyagawa, Hiromitsu Tanaka
1TANAKA Project, Center for Advanced Science and Innovation, Osaka University, 3-1 Yamadaoka, Osaka 565-0871, Japan.
Aim:
To characterize mouse capping protein alpha3 (CPalpha3) during spermatogenesis and sperm maturation.
Methods:
We produced rat anti-CPalpha3 antiserum and examined the expression of CPalpha3 in various mouse tissues using Western blot analysis and the localization of CPalpha3 in testicular and epididymal sperm using immunohistochemical analyses. We also examined how the localization of CPalpha3 and beta-actin (ACTB) in sperm changed after the acrosomal reaction by performing immunohistochemical analyses using anti-CPalpha3 antiserum and anti-actin antibody.
Results:
Western blot analysis using specific antiserum revealed that CPalpha3 was expressed specifically in testes. Interestingly, the molecular weight of CPalpha3 changed during sperm maturation in the epididymis. Furthermore, the subcellular localization of CPalpha3 in sperm changed dynamically from the flagellum to the post-acrosomal region of the head during epididymal maturation. The distribution of ACTB was in the post-acrosomal region of the head and the flagellum. After inducing the acrosomal reaction, the CPalpha3 and ACTB localization was virtually identical to the localization before the acrosomal reaction.
Conclusion:
CPalpha3 might play an important role in sperm morphogenesis and/or sperm function.
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