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[Synthesis of Sudan I artificial antigen]
Wei Yu1, Xiaohong Wang, Youxiang Zhou
1College of Food Science and Technology, Huazhong Agricultural University, Key Lab Food Security Estimate of Agriculture, Wuhan 430070, China. wudichina2001@yahoo.com.cn
Objective:
To prepare Sudan I-HS conjugates with the carrier protein for developing a new immune assay for Sudan I.
Methods:
Sudan I was actived by succinic anhydride and converted into Sudan I hemisuccinate (Sudan I-HS) as the hapten, then Sudan I-HS was linked to carrier protein bovine serum albumin (BSA) as immunogen, and ovalbumin (OVA) as coating antigen all by the active ester method.
Results:
The results of IR and HPLC-MS indicated that the hapten was synthesized successfully. The conjugation ratios of Sudan I-HS-BSA and Sudan I-HS-OVA confirmed by ultraviolet spectrum were 11 : 1 and 5 : 1, respectively.
Conclusion:
Antigens Sudan I-HS-BSA and Sudan I-HS-OVA were prepared successfully.
