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[Enhancing effect of antisense oligonucleotide targeting bFGF on apoptosis in hepatoma cells in vitro]
Jie-Lin Qi1, Ning Wu, Deng-Feng Zhou
1Department of Radiation Oncology, Shandong Tumor Hospital and Institute, Jinan 250117, China.
Objective:
To investigate the cell cycle changes of hepatoma cells and the effect of antisense oligonucleotide targeting bFGF on apoptosis in the hepatoma cells.
Methods:
The oligodeoxynucleotides were transfected with Lipofectin into hepatoma HepG2 cells. Inhibition of bFGF protein expression was assessed by confocal laser scanning microscopy and Western blot under the best condition of transfection of antisense oligonucleotide targeting bFGF, and the apoptosis in those cells was determined by flow cytometry. HepG2 cells were cultured in 24-well culture dish. The cultured cells were divided into 3 groups: group 1, the normal control group without any treatment; group 2, transfected with antisense oligonucleotide targeting bFGF; group 3, transfected with scrambled sequence targeting bFGF.
Results:
The results from confocal microscopy and Western blot showed an inhibition of expression of bFGF at different levels under the best condition of transfection with antisense oligonucleotide targeting bFGF. The treatment with antisense oligonucleotide of bFGF not only reduced the expression of bFGF revealed by confocal microscopy and Western blotting, but also increased the apoptosis in HepG 2 cells (P < 0. 01).
Conclusion:
Treatment with antisense oligonucleotide of bFGF inhibits expression of bFGF protein and increase apoptosis. bFGF may take part in apoptosis regulation of hepatoma cells and may be used as a target in the treatment of hepatocellular carcinoma.
Insights
Antisense oligonucleotide targeting basic fibroblast growth factor (bFGF) effectively inhibits bFGF protein expression in hepatoma cells. This inhibition significantly increases apoptosis, suggesting bFGF as a potential therapeutic target for hepatocellular carcinoma.
Area of Science:
- Molecular biology
- Cell biology
- Cancer research
Context:
- Hepatocellular carcinoma (HCC) is a significant global health concern.
- Basic fibroblast growth factor (bFGF) plays a role in cell proliferation and survival.
- Understanding cell cycle regulation and apoptosis in hepatoma cells is crucial for developing targeted therapies.
Purpose:
- To investigate the impact of antisense oligonucleotide targeting bFGF on hepatoma cell cycle.
- To evaluate the effect of bFGF inhibition on apoptosis in hepatoma cells.
- To explore bFGF as a potential therapeutic target for hepatocellular carcinoma.
Summary:
- Antisense oligonucleotide targeting bFGF was transfected into HepG2 hepatoma cells.
- Inhibition of bFGF protein expression was confirmed using confocal microscopy and Western blot.
- Flow cytometry demonstrated a significant increase in apoptosis in treated cells compared to controls (P < 0.01).
Impact:
- Demonstrates that antisense oligonucleotide targeting bFGF effectively reduces bFGF expression in hepatoma cells.
- Provides evidence that inhibiting bFGF promotes apoptosis in hepatoma cells.
- Suggests that bFGF is involved in the regulation of apoptosis and could be a viable therapeutic target for hepatocellular carcinoma.
