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Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
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Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
12:57

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Published on: December 21, 2017

Unexpected complexity of the budding yeast transcriptome.

Takashi Ito1, Fumihito Miura, Miyuki Onda

  • 1Department of Computational Biology, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa 277-8561, Japan. ito@k.u-tokyo.ac.jp

IUBMB Life
|July 24, 2008
PubMed
Summary
This summary is machine-generated.

Budding yeast, Saccharomyces cerevisiae, exhibits pervasive transcription, producing numerous noncoding RNAs from previously unknown genomic regions. These noncoding RNAs may play regulatory roles in gene expression.

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Last Updated: Jul 3, 2026

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Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae
09:12

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

Published on: February 27, 2026

Area of Science:

  • Genomics
  • Molecular Biology
  • Eukaryotic Gene Regulation

Background:

  • The Saccharomyces cerevisiae genome, containing approximately 6,000 genes, was previously considered well-annotated.
  • Recent high-throughput studies have uncovered a significant number of previously undescribed transcripts in yeast.
  • These novel transcripts are largely non-protein-coding and originate from both strands of intragenic and intergenic regions.

Purpose of the Study:

  • To investigate the phenomenon of pervasive transcription in Saccharomyces cerevisiae.
  • To characterize the nature and potential function of the newly discovered noncoding RNAs.
  • To establish budding yeast as a model organism for studying pervasive transcription and noncoding RNAs.

Main Methods:

  • Tiling array hybridization
  • cDNA sequencing
  • Bioinformatic analysis of genomic data

Main Results:

  • Identification of a large number of previously unknown transcripts in the yeast genome.
  • Demonstration that these transcripts are predominantly noncoding RNAs.
  • Evidence suggesting transcription from both DNA strands in various genomic locations.

Conclusions:

  • Pervasive transcription, generating numerous noncoding RNAs, is a likely intrinsic feature of eukaryotic genomes, including yeast.
  • Some noncoding RNAs may regulate gene expression through mechanisms like transcriptional interference or histone modification.
  • Budding yeast serves as a valuable model system for exploring the functional significance of pervasive transcription and noncoding RNAs.