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Related Concept Videos

Crystal Growth: Principles of Crystallization01:25

Crystal Growth: Principles of Crystallization

Crystallization is a phase transformation process in which crystals are precipitated from a supersaturated solution or formed from other sources. During crystallization, atoms or molecules arrange themselves into a well-defined, rigid crystal lattice to minimize energy.
Initiating crystallization involves manipulating the concentration of the solute and the temperature of the solution. Since crystal growth occurs when the ratio of concentration and solubility of the solute in the solvent – the...
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Recrystallization is a purification technique used to separate impurities from solid compounds. In this technique, no chemical reactions occur. Instead, it exploits physical properties only, specifically, the solubility differences between the desired compound and impurities, either at a single temperature or at different temperatures, and under other selected conditions. The solid-solution equilibrium (solubility equilibrium) of each component in the solution represents a binary phase...

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Growing Protein Crystals with Distinct Dimensions Using Automated Crystallization Coupled with In Situ Dynamic Light Scattering
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Concentration control for protein crystallization via a continuously-fed crystallization chamber.

Masano Sugiyama1, Shramik Sengupta, Paul Todd

  • 1Department of Chemical Engineering, University of Minnesota, 421 Washington Avenue SE, Minneapolis, MN 55455, USA. sugiy002@umn.edu

Lab on a Chip
|July 25, 2008
PubMed
Summary

A new crystallization chamber offers precise control over protein crystal formation, enabling detailed kinetic studies. This device facilitates the analysis of nucleation and growth phases, yielding key kinetic parameters for lysozyme crystallization.

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Area of Science:

  • Crystallization science
  • Biophysics
  • Chemical engineering

Background:

  • Controlling crystal nucleation and growth is crucial for protein crystallization.
  • Existing methods offer limited control over the kinetic pathways during crystallization.
  • Understanding crystallization kinetics aids in optimizing crystal formation for research and industrial applications.

Purpose of the Study:

  • To develop and demonstrate a continuously-fed crystallization chamber for kinetic path control.
  • To investigate lysozyme crystallization kinetics using the developed device.
  • To determine kinetic parameters for heterogeneous and homogeneous nucleation.

Main Methods:

  • Fabrication of a continuously-fed crystallization chamber.
  • Crystallization of lysozyme within the chamber.
  • Development of a lumped kinetic model.
  • Determination of heterogeneous nucleation kinetics parameters.

Main Results:

  • The device enabled kinetic path control through the crystallization phase diagram.
  • Heterogeneous nucleation exhibited faster kinetics than homogeneous nucleation.
  • Slower growth kinetics were observed for heterogeneous nucleation compared to homogeneous nucleation.
  • Lumped-model analysis successfully extracted kinetic parameters.

Conclusions:

  • The novel crystallization chamber provides enhanced control over the chemical environment for nucleation and growth studies.
  • The device allows for lumped-model analysis to extract critical kinetic parameters.
  • Findings contribute to a better understanding of protein crystallization kinetics and control.