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Updated: Jul 3, 2026

Mapping Dysfunctional Protein-Protein Interactions in Disease
Published on: October 24, 2025
Application of a peptide-based PF2D platform for quantitative proteomics in disease biomarker discovery
Hyoung-Joo Lee1, Min-Jeong Kang, Eun-Young Lee
1Department of Biochemistry, Yonsei Proteome Research Center and Biomedical Proteome Research Center, Yonsei University, Seoul, Korea.
Abstract:
A peptide-based 2-D liquid phase fractionation (PF2D) system was used in a quantitative proteomic analysis of hepatocellular carcinoma. 2-D liquid maps of peptide specimens showed better resolution than those of proteins, leading to the identification of differentially expressed proteins. Peptide-based PF2D gave well-matched theoretical and experimental pI values and was proven to be a very efficient and versatile analytical tool for both large-scale profiling and quantification of phosphoproteins in disease biomarker discovery.
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