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Related Experiment Video

Updated: Jul 3, 2026

Measuring and Modeling Contractile Drying in Human Stratum Corneum
08:00

Measuring and Modeling Contractile Drying in Human Stratum Corneum

Published on: March 1, 2017

Two new methods for preparing a unique stratum corneum substitute.

Daniël Groen1, Gert S Gooris, Maria Ponec

  • 1Leiden/Amsterdam Center for Drug Research, Department of Drug Delivery Technology, Gorlaeus Laboratories, University of Leiden, P.O. Box 9502, 2300 RA Leiden, The Netherlands.

Biochimica Et Biophysica Acta
|July 26, 2008
PubMed
Summary

New methods enhance the stratum corneum substitute (SCS), a synthetic skin barrier model. The linomat method offers superior lipid uniformity and reduced loss, making it ideal for skin permeation studies.

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Area of Science:

  • Dermatology
  • Materials Science
  • Biophysics

Background:

  • Stratum corneum lipids are crucial for skin barrier function.
  • An in vitro model, the stratum corneum substitute (SCS), using synthetic lipids, exists for permeation studies.
  • Improving SCS preparation efficiency and reproducibility is needed.

Purpose of the Study:

  • To develop and evaluate new preparation methods for the stratum corneum substitute (SCS).
  • To assess the properties and performance of SCS prepared by different methods.

Main Methods:

  • Development of two new SCS preparation methods: rotor airbrush and linomat methods.
  • Comparison with a manual airbrush method.
  • Investigation of SCS lipid composition, distribution, and organization.

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  • Evaluation of benzoic acid permeation through SCS.

    • Main Results:

    • SCS prepared by all methods exhibited uniform lipid composition and distribution.
    • Lipids in SCS formed crystalline lamellar phases, mimicking human stratum corneum.
    • Benzoic acid permeation profiles through SCS closely resembled those of human stratum corneum.
    • The rotor method improved efficiency and reproducibility over the manual method.
    • The linomat method minimized lipid loss and yielded more uniform membrane thickness.

    Conclusions:

    • The linomat method is the preferred technique for preparing stratum corneum substitutes.
    • The developed SCS models effectively mimic human stratum corneum for in vitro permeation studies.