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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Updated: Jul 3, 2026

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

Solid-state nanopore channels with DNA selectivity.

Samir M Iqbal1, Demir Akin, Rashid Bashir

  • 1Birck Nanotechnology Center, School of Electrical and Computer Engineering, Purdue University, West Lafayette, Indiana 47907, USA.

Nature Nanotechnology
|July 26, 2008
PubMed
Summary
This summary is machine-generated.

Solid-state nanopores functionalized with DNA probes selectively transport complementary single-stranded DNA (ssDNA). Even a single base mismatch significantly alters translocation, enabling precise DNA sequencing applications.

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Area of Science:

  • Nanotechnology
  • Biophysics
  • Molecular Biology

Background:

  • Solid-state nanopores are promising for next-generation DNA sequencing.
  • They offer advantages over biological nanopores, including environmental stability.
  • DNA sequencing involves measuring changes in forces and ion currents as DNA passes through a nanopore.

Purpose of the Study:

  • To develop solid-state nanopore channels selective for single-stranded DNA (ssDNA).
  • To investigate the interaction between functionalized nanopores and target ssDNA molecules.
  • To demonstrate the potential for high-resolution DNA detection using nanopore technology.

Main Methods:

  • Functionalization of solid-state nanopores with hairpin loop DNA probes.
  • Application of an electrical field to induce ssDNA transport.
  • Single-molecule measurements of molecular flux and translocation pulse duration.

Main Results:

  • Nanopores functionalized with DNA probes selectively transported complementary ssDNA.
  • A single base mismatch between probe and target resulted in longer translocation pulses.
  • A significant reduction in translocation events was observed with even a single base mismatch.

Conclusions:

  • Solid-state nanopores can be engineered for selective ssDNA transport.
  • Nanopore-based single-molecule measurements provide fundamental insights into channel-molecule interactions.
  • This approach offers a potential method for sensitive and specific DNA detection and sequencing.