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A highly conserved baculovirus gene p48 (ac103) is essential for BV production and ODV envelopment

Meijin Yuan1, Wenbi Wu, Chao Liu

  • 1State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.

Virology
|July 29, 2008
PubMed

Insights

Autographa californica multiple nucleopolyhedrovirus (AcMNPV) p48 gene is essential for virus propagation. Deleting p48 prevents budded virus production and nucleocapsid envelopment, crucial steps in the AcMNPV life cycle.

Area of Science:

  • Virology
  • Molecular Biology
  • Insect Pathology

Background:

  • The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) p48 (ac103) gene is conserved across baculoviruses but its function remains uncharacterized.
  • Understanding essential viral genes is critical for baculovirus research and applications.

Purpose of the Study:

  • To elucidate the function of the AcMNPV p48 gene in the viral life cycle.
  • To investigate the role of P48 in viral propagation, budded virus (BV) production, and occlusion-derived virus (ODV) formation.

Main Methods:

  • Generation of a p48 knockout mutant (vAc(P48-KO-PH-GFP)) in the AcMNPV bacmid.
  • Replication assays, titration, Western blotting, qPCR, electron microscopy, and confocal microscopy were employed.

Main Results:

  • The p48-null mutant failed to propagate in cell culture and produce infectious budded viruses (BVs).
  • Viral DNA replication was unaffected by p48 deletion.
  • P48 is essential for nucleocapsid envelopment into occlusion-derived viruses (ODVs) and subsequent occlusion.
  • P48 localizes to the nucleus center.

Conclusions:

  • The p48 gene is indispensable for AcMNPV propagation, specifically for BV production and ODV envelopment.
  • P48 plays a critical role in the late stages of the AcMNPV life cycle.

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