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In vitro Assessment of Myocardial Protection following Hypothermia-Preconditioning in a Human Cardiac Myocytes Model
Published on: October 27, 2020
Cardiac preconditioning with local laser-induced hyperthermia.
Bin Gong1, Brent Bell, Paul J Boor
1Center of Biomedical Engineering, Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-0456, USA.
The Journal of Surgical Research
|July 29, 2008
Summary
Local laser-induced hyperthermia effectively preconditioned cardiac tissue against myocardial infarction, offering a practical approach for tissue protection. This method demonstrated comparable results to whole-body hyperthermia in reducing infarct size and up-regulating heat shock proteins.
Area of Science:
- Cardiology
- Biomedical Engineering
- Therapeutic Hypothermia
Background:
- Whole body hyperthermia (WBH) effectively preconditioned cardiac tissue against reperfusion injury.
- The study investigated regional sublethal laser-induced hyperthermia for cardiac preconditioning.
Purpose of the Study:
- To assess the efficacy of regional laser-induced hyperthermia in preventing ischemic-induced myocardial infarction.
- To compare the protective effects of local hyperthermia with WBH.
Main Methods:
- Rat hearts were locally heated to 42°C on the left ventricle epicardial surface using diode laser irradiation for 20 minutes.
- Control groups received either whole-body hyperthermia (WBH) or sham treatment.
- Myocardial infarction was induced via coronary artery occlusion followed by reperfusion.
Main Results:
- Laser-induced local hyperthermia significantly reduced infarct size (15.4% +/- 3.1%) compared to the sham group (49.8% +/- 6.6%).
- The cardiac protection achieved by local hyperthermia was comparable to that of WBH.
- Both local hyperthermia and WBH similarly upregulated heat shock protein 70 in cardiac tissue, with local hyperthermia affecting regions beyond direct irradiation.
Conclusions:
- Sublethal local cardiac heating using diode laser irradiation enhances myocardial salvage.
- This technique presents a practical method for preconditioning cardiac tissue against reperfusion injury.
