Related Experiment Video
Updated: Jul 3, 2026
Murine Model of Wound Healing
Published on: May 28, 2013
Study of wound healing in rats treated with topical and injected mitomycin-C
Fernando de Andrade Quintanilha Ribeiro1, Janaína de Pádua Borges, Lusiele Guaraldo
1Otorhinolaryngology Department, Faculdade de Ciências Médicas, Santa Casa de São Paulo, Brazil.
Unlabelled:
Experimental study in animals.
Introduction:
Mitomycin C has been used as a fibroblasts inhibitor, thus reducing the scaring process in surgical wounds.
Aim:
This paper aims at assessing the use of Mitomycin C to reduce the scaring process through its topical use and later injected reinforcements.
Materials And Methods:
We used a model of a creating a wound in the dorsum of rats, removing a circular piece of skin and letting it heal by itself. We used 18 rats, divided in three groups. The first group - Control, the second with topical use, and a third group with injected mitomycin C reinforcement, monthly for 2 months. After 3 months the animals were slaughtered and the scars were surgically removed and sent for histology study.
Results:
We noticed, under different criteria, that healing with topical mitomycin is less intense; however, when it was injected, the parameters were again comparable to those from the control group.
Discussion:
We believe that injected mitomycin C in the scar, since it is highly toxic, it destroys tissue and brings about scar neoformation.
Conclusions:
Mitomycin C reduces the scaring process when used topically; however, it increases scar tissue formation when injected in these wounds.
